Cytochrome synthesis and its regulation in Spirillum itersonii.

Printed in Great Britain I so la t ion and Character iza t ion

The bacterium Spirillum itersonii has been used in studies of the regulation of cytochrome synthesis (Clark-Walker, Rittenberg & Lascelles, I967), but we felt that these studies would be aided if a transducing bacteriophage was available for genetic analysis. Attempts to isolate such a phage by induction with mitomycin C and ultraviolet light were unsuccessful, but examination of induced cultur...

Growth and cytochrome synthesis in a hemin-requiring mutant of Spirillum itersonii.

Spirillum itersonii forms considerable amounts of band c-type cytochrome when grown under low aeration, and we have suggested that synthesis of the heme prosthetic group, derived from glycine and succinyl CoA, might be important in regulating the formation of the complete hemoproteins (1). The isolation of a mutant strain requiring hemin (ferric protoporphyrin chloride) has enabled a more direc...

Base composition of deoxyribonucleic acid from Spirillum volutans, S. serpens and S. itersonii.

Purification and characterization of the membrane-bound ferrochelatase from Spirillum itersonii.

The membrane-bound enzyme ferrochelatase (protoheme ferro-lyase, EC 4.99.1.1) was purified from isolated membrane fragments of Spirillum itersonii approximately 490-fold. Purification was achieved by solubilization with chaotropic salts followed by ammonium sulfate fractionation, diethylaminoethyl-cellulose chromatography, and gel filtration on Sephadex G-200. The purified enzyme has an apparen...

Regulatory Circuits Controlling Photosynthesis Gene Expression

Carl E. Bauer and Terry H. Bird reduced (and protonated) it migrates to the cytochrome bc1 complex. The cytochrome bc1 complex then cycles Department of Biology Indiana University electrons from the quinone back to the reaction center via cytochrome c2 (or cytochrome cy). The bc1 complex Bloomington, Indiana 47405 also functions to translocate protons across the cytoplasmic membrane which creat...